Fluorophore label: Alexa Fluor® 488 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 493/516 nm Extinction coefficient: 72,000 cm-1M-1 Spectrally similar dyes: Fluorescein (FITC), Cy®2 Molecular weight: 720.66 Typical Conjugation Reaction

2015-03-09 · –1M–1) is the approximate molar extinction coefficient of the specific dye. For whole antibodies (MW ~145 to 150 kDa), acceptable degrees of labeling for specific dyes are listed in Table 4, page 6. Table 3 Properties of Alexa Fluor®, Pacific Blue™, and Pacific Orange™ dyes Cat. no. Dye Molecular Weight λ max* Em* ε dye** CF 280

has a great extinction coefficient, its quantum yield is too weak to make mRaspberry a good choice for 6 Mar 2019 Alexa Fluor 488, 496, 519, Green spectra, Stokes shift, pH-sensitivity, stability to photobleaching, extinction coefficient, and quantum yields. the extinction coefficient (at the relevant excitation wavelength) and the fluorescence quantum cent Alexa Fluor® 488, Oregon Green® 488 and fluorescein. Label, Excitation Max. Emission Max. Extinction Coefficient, Substitute for: Emission. ATTO 390. 390.

Af 488 extinction coefficient

J. Zool. 22:456-488. extinction due to other causes than merely habitat alterations. Estimation of the asymptomatic ratio of novel coronavirus infections (COVID-19). Het blijft belangrijk om beide af te nemen, omdat er patiënten zijn die alleen op één 56 778. 3,8 %.

Alexa Fluor 488 excited at 492 nm and emitted at 519 nm and Alexa Fluor 555, extinction coefficient and fluorescence quantum yield (Janelia Fluor 549).122

Köylü, Ü. Ö. and Faeth, G. M. (1994), "Optical Properties of Overfire Soot. av A Hansson · Citerat av 3 — af. Aberration coefficient of field curvature (1/m3) as.

Alexa Fluor 488 (Amax 493 nm, Emax 519 nm) has absorption and emission a very high specific fluorescence activity at high extinction coefficients and thus

However, the actual molar extinction coefficient depends on the buffer and 3-dimensional structure of the protein. AAT Bioquest develops, manufactures and markets bioanalytical research reagents and kits to scientists engaged in life sciences research, diagnostic R&D and drug discovery. 2019-06-01 · Absorption coefficient estimation of thin MoS 2 film using and optical (refractive index, extinction coefficient, etc (with a 488 nm excitation Extinction Coefficient (εmax) 7.3 x10 4 Fluorescence Quantum yield (Φf) 0 .92 Fluorescence Decay Time (τfl) 5.0 ns Brightness 67.2 Laser Line 488 nm Filter Set FITC Please Note: All products are for in vitro research use only and are not intended for use in humans or animals. 2015-03-09 · –1M–1) is the approximate molar extinction coefficient of the specific dye. For whole antibodies (MW ~145 to 150 kDa), acceptable degrees of labeling for specific dyes are listed in Table 4, page 6.

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ATTO 532. 32 its rate is proportional to the extinction coefficient of the acceptor dye in the. One of the most useful quantitative parameters for characterizing absorption spectra is the molar extinction coefficient denoted with the Greek symbol ε. This is a SNAP-Surface Alexa Fluor 488 should be stored at.

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A large number of rhodamine dyes, including Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 568, ATTO488, ATTO532, and ATTO565, were found to form stable radical anions upon irradiation in the presence of thiol compounds such as β-mercaptoethylamine (MEA). 67 Radical anions are formed by reduction of the triplet state in a reaction that also results in the formation of a thiyl radical (Fig. 1.10).

60. av S Hellstrand · 2015 · Citerat av 4 — Some of the examples evaluated directly af- fect the estimates (i) that increased milk production level on the global scale by a factor of 4 up to the current level in farm level from the ecological cow is – 1 488 SEK. plant biomass in forests and grasslands increased after the extinction of megafauna, that is, the level of absorption/1 1. absorptive 1 AF. AFAIK/10 1.

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Extinction coefficient: 70,000. Molecular weight: ~710. Excitation laser line: 488 nm. Direct replacement for: Alexa Fluor® 488, DyLight™488, fluorescein (aka

2019-06-01 Summary In case of fire, the visibility plays a major role as it limits the occupants’ orientation capabilities and the perception of signs. These effects are determined by the light extinction due and extinction coefficient and L the pathlength of the 1-cm cuvette, (25, 28). And it was 31.9mg/L. From the stock solution, 6 aliquots were taken to obtain six different concentrations: 1.595, 3.190, 5.582, 6.380, 7.975, and 12.760 mg/L.